Bio-Rad Sequi-Gen GT Sequencing Cell - Manual (Page 4)

Section 1
General Information
1.1 Introduction to the Sequi-Gen * GT Nucleic Acid Sequencing Cell
The Sequi-Gen GT cell is a modular electrophoresis cell capable of separating nucleic
acids with single base-pair resolution, using a vertical slab gel format. This manual tells you
how to operate and care for your new Sequi-Gen GT cell. Read Sections 1 through 3 before
attempting to assemble the cell. The remainder of the manual gives you detailed procedures,
a troubleshooting guide, and parts lists.
The Sequi-Gen GT cell employs a simple design that provides maximum resolution with
high reproducibility, while eliminating the temperature artifacts which often occur in sequenc-
ing gels. Some of the unique features of this sequencing cell are the gel casting method,
durable construction, modular components, and ease of operation, which make this the most
advanced DNA sequencing cell available.
Note:
This manual contains instructions for the Sequi-Gen GT electrophoresis systems only.
Prior to the release of the Sequi-Gen GT systems, Bio-Rad supplied two similar sequenc-
ing electrophoresis cell systems: the original Sequi-Gen cell and the Sequi-Gen II cell. This
manual does not provide information on these systems. Contact your Bio-Rad represen-
tatives for information on the original Sequi-Gen and the Sequi-Gen II systems.
* US Patent number 4,663,015 issued to Bio-Rad Laboratories.
Safety
The Sequi-Gen GT cell has safety features to protect the operator from injury. These
features include:
• Interlocking safety lids to prevent high voltage buffer shock
• Permanently sealed upper buffer chamber to prevent leaks and arcing
• Plastic components made from self-extinguishing material
• Full-length clamps to shield user from edges of glass plates
• Chemically tempered glass plates that significantly reduce glass plate breaking due to
overheating and routine heating and cooling
• No exposed metallic parts
• Pour spout in lower buffer chamber allows radioactive buffer to be easily and safely
poured for disposal
Important:
This apparatus meets I.E.C. 1010-1
† safety standards. Sequi-Gen GT systems are safe to
use when operated in accordance with the instructions. This instrument should not be
modified in any way. Alteration of this instrument will:
• Void the manufacturer’s warranty
• Void the IEC1010-1 safety certification
• Create a potential safety hazard
† IEC1010-1 is an internationally accepted electrical safety standard for laboratory instruments.
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