Bio-Rad Sequi-Gen GT Sequencing Cell - Manual (Page 11)

2.3 Electrical Path
Both electrode wires are positioned near the bottom of the gel. The upper buffer carries the
current from the cathode up to the top of the plates near the fill spout, where the gel is exposed.
The lower buffer contacts the gel at the bottom edge of the plates in the standard fashion
(See Figure 2.3).
Fig. 2.3. Electrical path through IPC (Integral Plate Chamber) to lower buffer reservoir.
Section 3
Cleaning and Maintenance
3.1 Cleaning and Siliconizing Plates
Important:
To insure “bubble-free” gels using the Sequi-Gen GT precision caster, the glass plates
must be thoroughly cleaned and the outer (long) glass plate siliconized or coated before
each use.
1. Clean both Sequi-Gen GT glass plates (IPC and outer plates) thoroughly before each use.
• Carefully place the plate into the sink and rinse with warm water.
• Pour powdered lab detergent (Alconox [Alconox, Inc.] or Micro [International
Products]) into a gloved hand and add sufficient water to make a paste.
• Apply the paste and scrub the entire glass surface with a gloved hand, using circular
motions.
8
Fill spout
Upper buffer
Polycarbonate
panel
Bonded glass
plate
Gel
Outer glass
plate
Silicone
adhesive bond
IPC
drain port
Lower
buffer