Bio-Rad Sequi-Gen GT Sequencing Cell - Manual (Page 10)

Fig. 2.2. Sequi-Gen GT nucleic acid electrophoresis cell.
2.2 Gel Reagents and Electrophoresis Buffers
For most DNA sequencing or nucleic acid separations, a 19:1 acrylamide:bis solution is
required. A 1x TBE (Tris, boric acid and EDTA) solution is the preferred electrophoresis
buffer. Reproducibility is affected by the quality of the gel and buffer reagents. A full line of
high quality polyacrylamide gel reagents and nucleic acid electrophoresis buffers is available
from Bio-Rad. Premixed reagents and buffers are also available and offer convenience, time
savings, and reproducible results. Each reagent and buffer is purified to meet rigorous quality
control standards. See Section 6.2 for ordering information.
Sequi-Gen
® GT
Sequencing Cell
7
GT Base
Leveling Feet
GT Bottom
Safety Cover
Stabilizer
Bar
IPC
(Integral
Plate
Chamber)
GT Lever
Clamp
GT Lever
Clamp
GT Top
Safety
Cover