Bio-Rad Components for Older Model Spot Cutter User Manual

Page 9

2.4

Installing the Bandpass Filter

1. The bandpass filter is shipped in the Filter Assembly box. It includes a filter holder, a

620/60 nm bandpass filter, and a 0.9 mm hex wrench.

Fig. 9. Lens and Bandpass filter set.

2. Screw the mounted filter into the filter holder. The threads are very fine, so be careful not

to cross the threads.

Fig. 10. Lens and filter on camera.

3. To attach the filter holder to the lens of the camera, slide the filter holder up over the lens

until stops. Using the 0.9 mm hex wrench, gently tighten the setscrew on the side of the
filter holder.

Note:

Bandpass Filter
The bandpass filter is used for both the white light (visible stain) and UV light (fluorescent
stain) modes. Once it is installed, it is not taken off the lens. The only adjustment to the
camera between the two light modes is a change in the aperture from the nearly closed
(f-stop 5.6

–16) position for white light, to the open (f-stop 1.4–2.8) position for UV lights.

Lens (included with
the spot cutter)

0.9 mm hex wrench

Filter

Filter holder