Bio-Rad Components for Older Model Spot Cutter - Manual (Page 2)

Table of Contents
S Se ec cttiio on n 1 1 IIn nttrro od du uc cttiio on n ......................................................................1
1.1 General Description ..........................................................1
1.2 Components Received ....................................................1
1.3 Common Re-order Parts ..................................................1
1.4 Physical Description ........................................................1
1.5 Specifications ..................................................................2
1.6 Safety ..............................................................................2
S Se ec cttiio on n 2 2 IIn ns stta alllla attiio on n ........................................................................3
2.1 Unpacking the Enclosure..................................................3
2.2 Remove the Door ............................................................4
2.3 Installing the UV Lights ....................................................4
2.4 Installing the Bandpass Filter ............................................6
2.5 Spot Cutter Installation ....................................................7
2.6 Installing the Enclosure Cabinet........................................7
2.7 Software Setup ................................................................7
S Se ec cttiio on n 3 3 S Se ettttiin ng gs s a an nd d C Ca alliib brra attiio on n ..................................................8
3.1 Setting Aperture for White and UV Lights Settings ..........8
3.2 Toggle Switch for White or UV Lights ..............................9
3.3 Checklist for Operation in UV Light Mode ......................10
3.4 Checklist for Operation in White Light Mode ..................11
3.5 Calibration ......................................................................11
S Se ec cttiio on n 4 4 F Fllu uo orre es sc ce en ntt O Op pe erra attiio on n iin n B Ba as siic c E Ex xc ciis siio on n T To oo oll ................12
4.1 Calibration and the UV Light Option ..............................12
4.2 Acquire Fluorescent Image in Basic Excision Tool ..........12
4.3 Cutting Spots From Fluorescent STained Gels in
Basic Excision Tool ........................................................13
4.4 Re-cuts in Basic Excision Tool........................................13
4.5 Begin/Resume and Pause Buttons ................................17
4.6 Confirm Cuts ..................................................................17
S Se ec cttiio on n 5 5 F Fllu uo orre es sc ce en ntt O Op pe erra attiio on n iin n IIn ntte eg grra atte ed d E Ex xc ciis siio on n T To oo oll ..........18
5.1 Calibration and the UV Light Option ..............................18
5.2 Acquire Fluorescent Image in Basic Excision Tool ..........19
5.3 Re-cuts in Integrated Excision Tool ................................20
5.4 Begin/Resume and Pause Buttons ................................24
5.5 Confirm Cuts ..................................................................24