Bio-Rad Components for Older Model Spot Cutter - Manual (Page 27)

16. Click on "Begin/Resume" after the selections are made. The “MTP Image“ window will
close. All the spots that have been marked for re-cut will now be cut.
5.4 Begin/Resume and Pause Buttons
The Begin cut button has been modified in name to be more descriptive of the different
operations that may be used in the spot cutter.
The Pause button will pause the cut run and then give you the option of stopping the cut run
completely or resuming the run. After clicking on "Pause", the spot cutter will continue the cut it is
working on through eject in the microtiter plate and wash wells (if selected) before stopping. The
Pause button will be used for the "Confirm cuts" operation, described next.
5.5 Confirm Cuts
The Confirm cuts button was added to help in checking the cut progress of a fluorescent stained
gel. With visible stained gels, it is easy to look in on the spot cutter to see that the cutting tip is
cutting right on the spot. However, since the spots are not visible without UV illumination in a
fluorescent gel, the "Confirm cuts" operation provides an on-screen method for monitoring the cut
run.
1. After starting the cut run in Integrated Excision Tool, let the cut run proceed for 5
–10 spot
cuts.
2. When you want to check on the cut run, click on "Pause".
3. Click on YES to "Confirm Pause run".
4. Click on the "Confirm cuts" button.
5. The camera will acquire a new image and create a floating window on top of the existing
screen.
6. This window will display the gel image with spot "holes" where it has been cut by the spot
cutter. It will also show the overlay circles of the cut targets. The white circles are the spots
that have already been cut, and the green circles are the spots yet to be cut.
FIg. 33. Integrated Excision Tool Confirm cuts function.
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