Bio-Rad Components for Older Model Spot Cutter - Manual (Page 13)

3.3 Checklist for Operation in UV Light Mode
1. Toggle the white/UV light switch on the back of the ballast box on the spot cutter to the
UV lights position.
2. Check the UV lights by pushing the red "Lights on" button on the lower front right side of
the spot cutter base. The white lights under the platform should NOT come on. If they do
come on under the platform, toggle the back switch to the correct position and re-check
the lights. The UV lights will not come on while the door is open because of the safety
interlock door switch.
3. Open the aperture setting to approximately f-stop 1.4 or the "UV / open" position.
4. Select the "UV on (Fluor)" lights setting in either the Basic Excision Tool or the Integrated
Excision Tool screen.
5. Manually enter an exposure time in the box marked “Time (secs)”. Typically 1 to 40 seconds.
Note:
UV lights Indicator Light
When the UV lights are active and the door is closed, the UV lights are confirmed to be
active with the external amber light on the side of the Enclosure nearest the door handle,
labeled "UV lights in operation". This light will turn on when the lights are actually in use
during image acquisition. The light will otherwise be off indicating that it is safe to open the
door. A safety interlock switch immediately stops power to the UV lights if the door is opened.
Note:
Fluorescent Target
Once the Enclosure is installed, and the light settings and aperture are ready for imaging, a
test image may be desirable with a known target. Included in the Enclosure is a Fluorescent
imaging target. It is the square, clear plastic target with lines, squares and
numbers on it.
When imaged with the UV lights, it should look like this on the computer screen:
Fig. 17. UV light exposure image of fluorescent target.
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