Bio-Rad Components for Older Model Spot Cutter User Manual
Page 3

Table of Contents
Page
Section 1
Introduction ..................................................................................................1
1.1 General
Description....................................................................................................1
1.2 Operation ....................................................................................................................1
1.3 Caution........................................................................................................................1
1.4
Moving The Spot Cutter.............................................................................................1
1.5 Computer
Recommendations.....................................................................................1
1.6
Cutting Excision Sizes ...............................................................................................1
1.7 Power
Setting .............................................................................................................1
Section 2
Getting Started .............................................................................................2
2.1 Preliminary
Instructions .............................................................................................2
2.2 Connector
Cables .......................................................................................................2
2.3 Software
Setup ...........................................................................................................3
2.4
Initial System Set Up..................................................................................................3
2.5
Quick Guide to Spot Excision....................................................................................3
Section 3
How To Use Photo Enhancer and the Digital Camera ............................4
3.1
Turning on the Digital Camera ..................................................................................4
3.2
Selecting the DC120 Digital Access (TWAIN Acquire) Window ...........................5
3.3 Camera
Functions.......................................................................................................5
3.4
Acquiring an Image....................................................................................................6
3.5
Saving an Image .........................................................................................................7
3.6
Erasing Picture Memory ............................................................................................7
Section 4
Selecting and Cutting Spots ........................................................................7
4.1
Opening an Image File Format ..................................................................................7
4.2
Using the Zoom Window...........................................................................................7
4.3
Selecting a Spot & MTP Location .............................................................................8
4.4 Deleting
Spots ............................................................................................................8
4.5
Making Cuts (Excisions)............................................................................................9
4.6
Re-Cut Previous Spot .................................................................................................9
Section 5
Working Surface ........................................................................................10
5.1 Excision
Mats ...........................................................................................................10
5.2 Cutting
Surface.........................................................................................................11
5.3
Gel Sizer and Frames for 2-D Gels..........................................................................11
5.4
Microtiter Plate (MTP) Holder ................................................................................11
Section 6
Calibration ..................................................................................................12
6.1 Camera
Alignment ...................................................................................................12
6.2 Calibration
Definitions.............................................................................................12
6.3 Calibration
Procedure...............................................................................................12
Section 7
The Gel Cutting System Menu Bar..........................................................16
7.1 File
Menu..................................................................................................................16
7.2 View
Menu ...............................................................................................................17
7.3 Cutting
Menu............................................................................................................18
7.4 Operation
Menu........................................................................................................19
7.5 Window
Menu..........................................................................................................21
7.6 Help ..........................................................................................................................21