Bio-Rad PROTEAN Plus Dodeca Cell - Manual (Page 6)

Ceramic cooling core Coolant from a refrigerated circulator passes
through the ceramic cooling core to cool the
buffer during the electrophoresis run. An
ethylene glycol:water (20:80) solution is
recommended as coolant.
Buffer Recirculation Pump The self-priming oscillating pump pulls buffer
from the top of the cell and refills from the
bottom of the cell through the buffer coil to
maintain a constant temperature in the cell.
Buffer coil The buffer coil sits on the bottom of the
Dodeca Cell. The small holes in the coil
release buffer around the ceramic cooling cores
and into the Dodeca Cell.
Buffer exhaust tube The buffer exhaust tube connects to the lid of
the Dodeca Cell via a quick-connect fitting.
The other end connects to the inlet of the
Buffer Recirculation Pump.
Electrode cards Plate electrodes are attached to the electrode
cards for easy removal. The anode is made
of platinum-coated titanium and the cathode
is made of stainless steel. DO NOT reverse
the direction of the current, as this will
damage the electrode.
Gasket Assembly This assembly contains the rubber gaskets that
hold the gel cassettes in position. The rubber
gaskets are mounted on an acrylic support for
easy removal. The tank has three positions for
the gasket assemblies: a single position on the
anode side and two positions on the cathode
side to accommodate different gel sizes.
Manifold The manifold in the lid is part of the buffer
recirculation system.
Manifold tubing The manifold tubing is required for proper buffer
recirculation. The manifold tubing is eleven
pieces of Tygon tubing that attach to the black
fittings of the manifold in the lid of the Dodeca
cell. The manifold tubing is necessary when
running Ready Gel precast gels or handcast gels
in PROTEAN II plates. These plates are shorter
and therefore the buffer level is lower in the tank.
Since the buffer level does not reach the black
fittings, the manifold tubing is required to extend
into the buffer so it can be properly recirculated.
Note: ALL eleven pieces of tubing must be used
when running any number of precast or handcast
PROTEAN II gel cassettes.
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