Bio-Rad PROTEAN Plus Dodeca Cell - Manual (Page 17)

Problem Cause Solution
5. SDS is precipitating.
a. Running buffer is too cold. a. Set the refrigerated
circulator to maintain a
buffer temperature of
approximately 20 ºC.
6. Agarose slides out a. The agarose is less a. Carefully measure the
of the cassette then 1.0%. agarose powder to 1.0%.
inside the cell.
7. Buffer in the anode side a. An electrochemical a. We recommend using
of the tank turns yellow reaction is taking place– fresh buffer for each run.
after a few runs. a component in the
Tris buffer is oxidized.
This reaction always
occurs during
electrophoresis, but
with the Dodeca cell
the large surface area of
the plate electrodes
intensifies this reaction
and thus causes the
buffer to turn yellow
very quickly.
Section 6
Product Information and Accessories
PROTEAN Plus Dodeca Cell
Catalog
Number Description
165-4150 PROTEAN Plus Dodeca Cell, 100/120V, includes tank and lid,
buffer recirculation pump with tubing.
165-4151 PROTEAN Plus Dodeca Cell, 220/240V,, includes tank and lid,
buffer recirculation pump with tubing.
165-4153 Replacement Tubing Kit, complete, includes all tubing required to
set up the buffer recirculation pathway.
165-4154 Replacement Gasket Assembly
165-4155 Electrode Card: ANODE
165-4156 Electrode Card: CATHODE
165-4157 Replacement Lid
165-4166 Manifold tubing, qty 11
165-4167 Buffer Exhaust Tubing, includes quick-connect fittings.
165-4158 Buffer Recirculation Pump, 100/120V
165-4159 Buffer Recirculation Pump, 220/240V
165-3320 Gel Releaser Tool, 5
Tygon is a registered trademark of Norton Co.
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