Bio-Rad PROTEAN Plus Dodeca Cell - Manual (Page 10)

Standard running buffer formulations:
Tris/Glycine/SDS
25mM Tris-Base (M.W. 121.1), 192mM Glycine (M.W. 75.07), 0.1% SDS, pH 8.3
(Do not adjust the pH with acid or base. If the pH is not accurate remake the buffer).
Tris/Tricine/SDS
100mM Tris-Base (M.W. 121.1), 100mM Tricine (M.W. 179.2), 0.1% SDS, pH 8.3
(Do not adjust the pH with acid or base. If the pH not accurate remake the buffer).
Premixed buffers are available (see section 6). Mix 1 liter of the premixed 10x buffer
with 9 liters of distilled water to prepare 10 liters of 1x running buffer.
3.2 Gel Preparation
All gels in a single electrophoresis experiment must use the same electrophoresis buffer
system. If running gels with different % acrylamide, run times between gels may vary slightly.
Please refer to the PROTEAN Plus multi-casting chamber and the Model 495 Gradient Former
instruction manuals for instructions on handcasting gels in the PROTEAN Plus hinged spacer
plates.
Reminder: 16 x 16 cm gels are not recommended to run in the PROTEAN Plus Dodeca Cell.
3.2.1 Orientation of gels inside the cell
Orientation inside the Dodeca Cell is identical, regardless of which type of plate is used.
With the PROTEAN Plus Dodeca Cell, the gel cassette is rotated 90° so that the hinged side
sits near the bottom of the tank. If PROTEAN II Ready Gel precast or handcast gels are run,
the greased side sits near the bottom of the tank.
_
+
SPACERS
HINGE or
GREASE
INSERT INTO
BUFFER TANK
8