Bio-Rad PROTEAN Plus Dodeca Cell User Manual

Page 13

When running PROTEAN II Ready Gels or 20 x 20 cm PROTEAN II gels, install the

eleven pieces of manifold tubing onto the black fittings in manifold in the lid. The manifold
tubing is not required when running the PROTEAN Plus hinged spacer plates, or other large
plates (greater than 26.5 cm wide), because these plates reach the height of the black fittings.
Once the manifold tubing is attached position the pieces of tubing in between the gel cassettes
so that it extends into the buffer (Figure 3.4B).

Note: ALL eleven pieces of tubing must be used when running any number of precast or
handcast PROTEAN II gel cassettes. Using less than eleven pieces of tubing may decrease the
cooling efficiency of the system and adversely affect the results.

Fig. 3.4B. Schematic diagram illustrating the proper use of the manifold tubing.

Place the lid on the tank and make sure the recirculation pump tubing is connected to the

manifold in the lid via the quick connect fitting. Set the Buffer Recirculation Pump to the
maximum setting (100) and turn it on. Check to make sure all the quick connect fittings are
tight and secure and that no buffer is leaking out.