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Bio-Rad PROTEAN Plus Dodeca Cell User Manual

Page 15

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Pick up the gel by gently lifting the bottom of the gel first with two hands and then

slowly continue lifting upward until the gel is completely off the plate (Figure 3.6C).

Transfer the gel to a

staining container.

Alternatively, a clean

and dry separation sheet
can be used to help lift the
gel from the plate. Place a
separation sheet on top of
the gel. Press down

Fig. 3.6C. Gently lift the gel from the plate.

on the separation sheet so that the gel sticks to the sheet.
Start at one of the bottom corners and slowly lift up the corner
of the separation sheet and gel. Hold the corner of the gel
against the corner of the separation sheet and then roll the
two together diagonally towards the opposite upper corner
(Figure 3.6D) the plate continue lifting the remainder of the
gel straight off the plate and transfer the gel to a staining
container.

Fig. 3.6D. Use a separation
sheet to lift the gel from the
plate.

Section 4
Maintenance

The PROTEAN Plus Dodeca Cell tank, gaskets, electrode cards and lid should be rinsed

after each use. For long term storage, flush all parts thoroughly with water to completely
remove any residual buffer.

For best performance, fresh buffer should be prepared for each run.

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