Bio-Rad PROTEAN Plus Dodeca Cell - Manual (Page 3)

Section 1
General Information
1.1 Introduction
The PROTEAN Plus Dodeca Cell* is a multi-cell for polyacrylamide gel electrophoresis.
It is primarily intended to run immobilized pH gradient (IPG) strips for the second dimension
of 2-D electrophoresis. It runs one to twelve large format gels simultaneously. The
PROTEAN Plus Dodeca cell system includes the Buffer Recirculation Pump that aids in
maintaining a constant temperature during the electrophoresis experiment. This system uses
the new PROTEAN Plus hinged spacer plates. These plates are strategically sealed at one
side with a silicon strip to create a hinged cassette. The hinge is required to prevent current
leakage and to achieve optimal results.
Specifications of the PROTEAN Plus Dodeca Cell
Tank and lid Acrylic
Anode Platinum-coated titanium plate electrode
Cathode Stainless steel plate electrode
Drain line Braided PVC
Drain line connectors Polypropylene
Cooling core Ceramic
Cooling core connector tubing Braided PVC
Manifold tubing Tygon tubing 1/4" ID (required for PROTEAN II
precast and handcast gels)
Buffer coil Acrylic
Gaskets Silicone rubber
Maximum buffer volume 22.5 liters (plate width: 27cm)
Minimum buffer volume 16.8 liters (plate width: 20cm)
Overall size (LxHxW)
Tank and lid 39.5 x 34.5 x 34.5 cm
Pump 19.5 x 12 x 17.5 cm
Precast gel compatibility PROTEAN II Ready gel precast gels with IPG comb
Handcast gel compatibility PROTEAN Plus hinged spacer plates
PROTEAN II 20 x 20 cm glass plates
Hinged spacer plate dimensions 26.8 x 22.5 cm; 26.8 x 20.5 cm
Slab gel dimension 25 x 20.5 cm; 20 x 20.5 cm; 18.5 x 20 cm; or
18.3 x 19.3 cm
Power safety limits 1000V, 250W
Weight 17.5 kg
*Patent pending
1