Bio-Rad Mini-PROTEAN® Tetra Cell - Manual (Page 34)

30
Troubleshooting Guide (cont.)
Problem Cause Solution
Poor end well
formation
Incorrect catalyst
formation
Monomer solution
not degassed.
Oxygen inhibits
polymerization
Prepare fresh catalyst solution,
or increase the catalyst
concentration of the stacking
gel to 0.06% APS and 0.12%
TEMED
Degas monomer solution
immediately prior to casting the
stacking gel
Webbing/excess
acrylamide behind
the comb
Incorrect catalyst
concentration
Prepare fresh catalyst solution,
or increase the catalyst
concentration of the stacking
gel to 0.06% APS and 0.12%
TEMED
The pressure cams
on the casting frame
are difficult to close
or make noise when
closed
Powder residue has
built up at the pivot
of the pressure cams
Rinse or wipe off the powder
residue before each use
*Polyacrylamide gels are described by reference to two
characteristics:
1. The total monomer concentration, (%T) and
2. The crosslinking monomer concentration (%C).
g acrylamide + bis-acrylamide x 100%
Total Volume
g bis-acrylamide x 100%
g acrylamide + bis-acrylamide