Bio-Rad Mini-PROTEAN® Tetra Cell - Manual (Page 24)

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Adjust to pH 8.8 with 6 N HCl. Bring total volume to 150 ml
with deionized water and store at 4°C. Alternatively, 1.5 M
Tris-HCl, pH 8.8 (1 L) premixed buffer can be used (Bio-Rad
catalog #161-0798).
4. 0.5 M Tris-HCl, pH 6.8
6 g Tris base
60 ml deionized water
Adjust to pH 6.8 with 6 N HCl. Bring total volume to 100 ml
with deionized water and store at 4°C. Alternatively, 0.5 M
Tris-HCl, pH 6.8 (1 L) premixed buffer can be used (Bio-Rad
catalog #161-0799).
5. Sample buffer (SDS reducing buffer)
3.55 ml deionized water
1.25 ml 0.5 M Tris-HCl, pH 6.8
2.5 ml glycerol
2.0 ml 10% (w/v) SDS
0.2 ml 0.5% (w/v) Bromophenol Blue
9.5 ml total volume
Store at room temperature.
Use: Add 50 µl ß-mercaptoethanol to 950 µl sample buffer
prior to use. Dilute the sample at least 1:2 with sample buffer
and heat at 95°C for 4 min.
6. 10x electrode (running) buffer, pH 8.3 (makes 1 L)
30.3 g Tris base
144.0 g glycine
10.0 g SDS
Dissolve and bring total volume up to 1,000 ml with deionized
water. Do not adjust pH with acid or base. Store at 4°C. If
precipitation occurs, warm to room temperature before use.
Alternatively, electrophoresis running buffer 10x Tris/glycine/
SDS, 5 L cube (Bio-Rad catalog #161-0772) can be used.
Use: Dilute 50 ml of 10x stock with 450 ml deionized water for
each electrophoresis run. Mix thoroughly before use.
7. 10% (w/v) APS (fresh daily)
100 mg ammonium persulfate
Dissolve in 1 ml of deionized water.