Bio-Rad Mini-PROTEAN® Tetra Cell - Manual (Page 16)

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2. Sample Loading
a. Fill the assembly (upper chamber) with buffer to just under
the edge of the outer gel plate.
b. Load samples into each of the assemblies while they are
sitting on a flat surface, outside of the tank.
c. Load the samples into the wells with a Hamilton syringe or a
pipet using gel loading tips.
d. If using Bio-Rad’s patented sample loading guide, place
it between the two gels in the electrode assembly. Sample
loading guides are available for 9, 10, 12, and 15-well
formats.
e. Use the sample loading guide to locate the sample wells.
Insert the Hamilton syringe or pipet tip into the slots of the
guide and fill the corresponding wells.
Note: Load samples slowly to allow them to settle evenly on the
bottom of the well. Be careful not to puncture the bottom of the
well with the syringe needle or pipet.
Note: Samples may be loaded in the modules prior to placing
the modules into the tank. Samples may also be loaded in the
modules after the modules have been placed into the tank. Both
methods will produce acceptable results. In both instances, the
assembly (upper chamber) and the tank (lower chamber) should
be filled with buffer according to the instructions under 2.2.2a
and 2.2.3d.
3. Placement of the Electrode Assemblies in the
Mini-PROTEAN Tetra Tank
Note: required total buffer volume, 700 ml for 2 gels; 1000 ml
for 4 gels.
The Mini-PROTEAN Tetra tank has two positions in which to
place two assemblies: the electrode assembly (back position)
and the companion running module (front position).
a. Begin by placing the tank on a flat surface, with the front of
the tank facing you (the front of the tank is the face that has
the 2-Gels and 4-Gels line markings); when oriented
properly, the red marking on the top inside edge of the tank
will be on your right, and the black marking on the top
inside edge of the tank will be on your left.