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Bio-Rad Mini-PROTEAN® Tetra Cell User Manual

Page 18

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14

6. Gel Removal

a. After electrophoresis is complete, turn off the power supply

and disconnect the electrical leads.

b. Remove the tank lid and carefully lift out the electrode

assemblies. Pour off and discard the running buffer.

Note: Always pour off the buffer before opening the arms of the

assembly, to avoid spilling the buffer.

c. Open the arms of the assembly and remove the gel cassettes.

d. Remove the gels from the gel cassette by gently separating

the two plates of the gel cassette.

Note: To remove the gel from a Ready Gel cassette, first slice

the tape along the sides of the Ready Gel cassette where the

inner glass plate meets the outer plastic plate.

e. Remove the gel by floating it off the plate by inverting the gel

and plate under fixative or transfer solution, agitating gently

until the gel separates from the plate.

f. Rinse the Mini-PROTEAN Tetra cell electrode assembly,

clamping frame, and mini tank with distilled, deionized water

after use.