Bio-Rad Mini-PROTEAN® Tetra Cell - Manual (Page 3)

Table of Contents
Section 1 General Information 1
1.1 Introduction 1
1.2 Components 1
1.3 Specifications 4
1.4 Safety 5
Section 2 Setup and Basic Operation 6
2.1 Gel Cassette Preparation 6
2.2 Electophoresis Module Assembly and
Sample Loading 9
Section 3 Separation Theory and Optimization 15
3.1 Introduction 15
3.2 SDS-PAGE (Laemmli) Buffer System 16
3.3 Native PAGE 17
Section 4 Reagent Preparation and Stock Solutions 19
4.1 Volumes Required per Gel 19
4.2 SDS-PAGE (Laemmli) Buffer System 19
4.3 Discontinuous Native PAGE (Ornstein-Davis) 22
4.4 Continuous Native PAGE 24
Section 5 References 27
Section 6 Maintenance 27
Section 7 Troubleshooting Guide 28
Section 8 Product Information and Accessories 31
Section 9 Warranty Information 35