Bio-Rad Mini-PROTEAN® Tetra Cell - Manual (Page 13)

9
Ready Gel ® Precast Gels
1. Ready Gel Cassette Preparation
Note: The Mini-PROTEAN Tetra cell is guaranteed for use with
Bio-Rad’s Ready Gel and Mini-PROTEAN ® precast gels. For
more information, go to www.bio-rad.com/mpgels.
a. Remove the Ready Gel from the storage pouch.
b. Gently remove the comb and rinse the wells thoroughly with
distilled water or running buffer.
c. Cut along the dotted line at the bottom of the Ready Gel
cassette with a razor blade.
d. Pull the clear tape at the bottom of the Ready Gel cassette
to expose the bottom edge of the gel.
e. Repeat for second Ready Gel.
Note: If only one or three gels are to be run, use the mini cell
buffer dam.
2.2 Electrophoresis Module Assembly and Sample Loading
Required materials:
• Clean and dry Mini-PROTEAN Tetra cell tank
• Electrophoresis module (electrode assembly module only for
1 or 2 gels; for 3 or 4 gels also use the companion running
module)
• Running buffer (700 ml for 2 gels; 1000 ml for 4 gels)
• Ready Gel precast gels or hand-cast gels
• PowerPac™ Basic power supply
1. Assembly
Note: When running 2 gels only, use the electrode assembly (the
one with the banana plugs), not the companion running module
(the one without the banana plugs). When running 4 gels, both
the electrode assembly and the companion running module
must be used, for a total of 4 gels (2 gels per assembly).