Bio-Rad Mini-PROTEAN® Tetra Cell - Manual (Page 11)

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f. Repeat steps a–e for additional gels.
Fig. 3. Assembling the Mini-PROTEAN casting stand and frame.
2.0 Gel Casting
a. Discontinuous Polyacrylamide Gels
i. Place a comb completely into the assembled gel cassette.
Mark the glass plate 1 cm below the comb teeth. This is the
level to which the resolving gel is poured. Remove the comb.
ii. Prepare the resolving gel monomer solution by combining all
reagents except APS and TEMED. (Refer to section 4 for
gel formulations.) Degas the solution under vacuum for at
least 15 min. Do not use a sink water aspirator.
iii. Add APS and TEMED to the degassed monomer solution
and pour to the mark using a glass or disposable plastic
pipet. Pour the solution smoothly to prevent it from mixing
with air.
3a 3b
3c 3d