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2 conditioning, Conditioning – Metrohm 850 Professional IC Anion MCS Gradient User Manual

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3.2 Conditioning

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850 Professional IC –Anion – MCS – Gradient

4 Setting the contact pressure of the peristaltic pump

Note

This work step needs to be performed only if a peristaltic pump is
being used.

If peristaltic pumps are used, set the contact pressure (see "Set
flow rate", page 53)
.

5 Rinsing the instrument without columns

Rinse the instrument (without columns) with eluent for 5 minutes.

The instrument is now prepared for the installation of the columns (see
Chapter 2.21, page 61)
.

3.2

Conditioning

After the installation and after switching on the instrument, the system
must be conditioned with eluent until a stable baseline is reached.

Note

After a change of eluent (see Chapter 4.4.2.3, page 71), the condi-
tioning time can lengthen considerably.

Conditioning the system

1 Preparing the software

Caution

Ensure that the flow set is not higher than the flow permissible for
the corresponding column (see column information sheet and chip
data set).

Start the PC program MagIC Net.

Open the Equilibration tab in MagIC Net.

Select (or create) a suitable method.