Leica Biosystems VT1000 P - Manual (Page 21)

21 Leica VT1000 P
4. Setup the Instrument
43
Fig. 25
4.7.2 Assembling the directional specimen holder fixture
The directional specimen holder fixture allows
for simple position correction of the specimen
surface when the specimen is glued in place.
• To do so, first remove the specimen clamp
(34, Fig. 16 ) by following the reverse order of
steps as described in Chap. 4.7 .
• Unscrew the knurled screw (38) completely.
You can now take the specimen clamp (40)
off of the base plate (39) ( Fig. 23 ).
• Attach the directional specimen holder fix-
ture (42) to the base plate so that the pin (41)
fits into the bore (45) provided.
• Then, screw the knurled screw (38) into the
thread and tighten it, thus fastening the di-
rectional specimen holder fixture (41) to the
base plate (39).
• Install it in the Vibratome tray (23) as de-
scribed in Chap. 4.7 (Fig. 25).
Fig. 23
38
39
40
39
42
41
45
Fig. 24
38
44
• The specimen to be sectioned (44) is glued
to the round specimen plate using the cy-
anoacrylate adhesive provided.
• To orient the specimen, slightly loosen the
knurled screw (43) on the right side, adjust to
the desired position and retighten the screw.
44 43