Leica Biosystems CM1850 UV User Manual
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Leica CM1850 – Cryostat
Table of contents
7.
Daily use of the instrument ................................................................................................................. 26
7.1
Specimen freezing ...................................................................................................................................... 26
7.1.1
Quick freeze shelf ........................................................................................................................................ 26
7.1.2
Stationary heat extractor ........................................................................................................................... 26
7.2
Specimen discs ........................................................................................................................................... 27
7.2.1
Inserting the specimen discs in the specimen head ............................................................................ 27
7.2.2
Specimen orientation ................................................................................................................................. 27
7.3
Sectioning ..................................................................................................................................................... 28
7.3.1
Trimming the specimen .............................................................................................................................. 28
7.3.2
Section thickness setting ........................................................................................................................... 28
7.4
Temperaturew selection chart (in minus °C) ........................................................................................ 29
7.5
Defrosting ..................................................................................................................................................... 30
7.5.1
Automatic defrosting of the cryochamber ............................................................................................. 30
7.5.2
Manual defrosting of the cryochamber .................................................................................................. 30
7.5.3
Manual defrosting of the quick freeze shelf .......................................................................................... 31
7.6
Terminating work ........................................................................................................................................ 31
7.6.1
Terminating daily work ............................................................................................................................... 31
7.6.2
Shutdown for a longer period ................................................................................................................... 32
8.
Troubleshooting .................................................................................................................................... 33
8.1
Error messages in the display ................................................................................................................... 33
8.2
Temperature control button ...................................................................................................................... 33
8.3
Possible causes and remedies ................................................................................................................. 34
9.
Cleaning, disinfection, maintenance ................................................................................................ 39
9.1
Cleaning ........................................................................................................................................................ 39
9.2
Spray disinfection with Leica Cryofect ................................................................................................... 40
9.3
Maintenance ................................................................................................................................................ 41
9.3.1
General maintenance ................................................................................................................................. 41
9.3.2
Removal of the microtome ......................................................................................................................... 42
9.3.3
Removal of the microtome cover ............................................................................................................. 44
9.3.4
Reinstallation of the microtome ................................................................................................................ 44
9.3.5
Replacement of the fuses .......................................................................................................................... 46
9.3.6
Replacement of the lamp ........................................................................................................................... 46
10.
Ordering information, optional accessories .................................................................................... 48
10.1
Ordering information ................................................................................................................................... 48
10.2
Optional accessories .................................................................................................................................. 50
10.2.1 Mobile heat extractor ................................................................................................................................. 50
10.2.2 Thermal block .............................................................................................................................................. 50
11.
Warranty and service .......................................................................................................................... 51
12.
Decontamination Certificate
(master) ...........................................................................................................
52