Leica Biosystems CM1850 UV - Manual (Page 36)

36 Instructions for use V 2.6 – 10/2012
Problem Cause Remedy
- Knife profile inappropriate for
the specimen to be cut.
- Incorrect clearance angle.
- Brush, forceps and/or cloth are
too warm.
- Plate too high above the cut-
ting edge. The adjustment was
carried out in the direction of
the cutting edge.
- Temperature incorrect for the
tissue cut.
- Knife profile inappropriate for
the specimen cut.
- Ice buildup in the knife back.
- Handwheel speed not uni-
form.
- Knife/blade not clamped tightly
enough.
- Specimen holder not clamped
tightly.
- Cryocompound applied to cold
specimen disc; specimen de-
tached from the disc after
freezing.
- Blunt cutting edge.
- Incorrect clearance angle.
- Microtome not properly dried
before reinstallation.
- Dried specimen.
- Anti-roll plate is too warm or
incorrectly positioned.
- Static electricity.
Condensation on anti-roll plate
and knife during cleaning
Anti-roll plate damage after ad-
justment
Thick-thin sections
- Use knife with different profile.
- Set correct angle.
- Store all tools on shelf in the
chamber.
- Raise plate when aligning.
- Be more careful next time.
- Select correct temperature.
- Wait until the correct tempera-
ture is reached.
- Use knife with different profile
(c or d).
- Remove ice.
- Adapt speed.
- Check knife/blade clamping.
- Check clamping.
- Apply cryocompound on warm
disc; mount specimen and
freeze.
- Use different part of the cutting
edge or replace the knife/
blade.
- Set correct angle.
- Dry microtome thoroughly.
- Prepare new specimen.
- Cool down anti-roll plate or
reposition plate.
- Remove static electricity.
8. Troubleshooting
Tissue sticks or crumbles on the
anti-roll plate