Techne TC-PLUS - Manual (Page 38)

36
OLIGO T
M
CALCULATOR
The oligo T
m
calculator can be used to estimate the T
m
of the PCR primers and help in selecting an
appropriate annealing temperature for the thermal cycling program.
• Touch Enter The Oligo Sequence in order to input
the sequence of the primer.
• Touch the A C G T buttons to enter the primer
sequence.
• Touch DEL to delete the last character.
• Touch CLR to delete the whole sequence.
• Use the arrow keys to scroll through the sequence.
• Touch OK to return to the calculator screen.
• Enter the primer concentration in the PCR mix.
• Enter the monovalent salt concentration of the PCR
mix.
• Touch Calculate to calculate the T m
of the primer.