Luminex MasterPlex CT Tutorial Manual - Manual (Page 8)

http://www.miraibio.com Plex GT 8
The Parameter Setting window
allows the user to insert the Allele
Call parameters:
ƒ Minimum Events
ƒ Relative Intensity (RI)
ƒ Intensity or (MFI)
The RI based Allele call uses two parameters: RI and Intensity Threshold.
The Intensity based Allele call just uses the Intensity Threshold.
For this tutorial, we will just use the default values of 20 Minimum Events, 25%
Minimum Reportable RI, and 35 Minimum Reportable MFI. To use these settings for all
loci, click on the Apply to all groups (loci) button. Since the organism for this data file
is diploid, please click on the Diploid radio button next to Ploidy and press the Apply
this Ploidy to all groups (loci) button. The Call Parameters are now set for Allele
Calling. Press the OK button to exit the Parameter Setting window.
Note: Bead counts lower than the minimum threshold will be highlighted in red in the
Bead Count view of the Typing Table.
Master