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Ocean Optics CHEM2000 User Manual

Page 31

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3: Experiment Tutorial

2. Select

Spectrometer | Kinetics Configuration from the menu to open the Kinetics Configuration

dialog box.

3. Enter

a

Preset Duration value to set the length of time for the entire kinetics process. Be sure to

select hours, minutes and seconds. The duration of your kinetics experiment cannot exceed 24
hours.

4. Enter

a

Preset Sampling Interval value to set the frequency of the data collected in a kinetics

process. Be sure to select hours, minutes and seconds. Data from a timed acquisition is stamped
with a time that is accurate to 1 millisecond.

5. Under

Wavelength, enter the single wavelengths from which you wish to collect data. You can

collect data from up to 4 single wavelengths, characterized as a, b, c, and d.

6. If you want the data graphed from these single wavelengths, or from the mathematical

calculations (described in the next step), select the Display box to the right of your values.

7. In the boxes next to

x =

and

y =

, you have the opportunity to perform calculations on the data

collected from the single wavelengths you specified as

a

,

b

,

c

, and

d

. In the

y =

box, you can also

use

x

, which represents the calculation used in

x =

.

8. Click

OK to confirm the parameters and close the dialog box.

9. Click

the

Scan button to begin the kinetics experiment. Make sure that Continuous is selected.

The top half of the graph displays a real-time full wavelength spectrum. The bottom half of the
graph displays the data for the selected wavelengths and their derived arithmetic calculations.
Each data set is stored with a time stamp.

10. Click the Stop button to stop the experiment. However, if a Preset Duration time was selected,

the experiment will automatically stop after the designated time has passed.

11. Select File | Save Kinetics Values from the menu to save a tab-delimited ASCII file with the

spectrometer’s serial number, active channel and acquisition parameters in a header. This file can
be opened with any text or spreadsheet editor.

105-00000-CHM-02-0405

PRELIMINARY DRAFT

25