Ocean Optics CHEM2000 - Manual (Page 19)

2: Using OOIChem Software
Show Legends
Select Edit | Show Legends to enable or disable the legends for the spectral trace,
overlays, and kinetics traces. When the legends are displayed, you can opt to
configure the traces by simply clicking on the legend trace you want to configure.
You have the opportunity to choose from several aesthetic functions such as: the
plot design of the spectrum, the point style used in the spectrum, the line style and
width desired, color of the plot, and a bar plot design. You can also choose to fill the
baseline in the spectrum. Utilize this function to differentiate one spectral trace from
another.
Spectrometer Menu Functions
Scan
When the Single mode is selected in the display screen, the Scan menu function acts as a snapshot. After
selecting the Single mode, select Spectrometer | Scan from the menu to take one scan of the sample.
When the Continuous mode is selected in the display screen, select Spectrometer | Scan from the menu
to continuously take scans.
Select Concentration Wavelength
This function is used when calculating the unknown concentration of a substance in a solution. You select
this function after you take an absorbance measurement of a standard solution with a known
concentration. Choose the wavelength of the highest peak in your absorbance spectrum. Then select
Spectrometer | Calculate Calibration Curve from the menu and complete the rest of your concentration
experiment. See
Concentration Experiments
for step-by-step instructions on calculating concentrations.
Calculate Calibration Curve
Concentration is the amount of a specified substance in a solution. In order to calculate concentration, you
must take absorbance measurements of a series of solutions with different known concentrations. The
length of the sample and the wavelength chosen for monitoring the amount of light absorbed are
constants. Then a linear plot from taking these scans is obtained. This Calibration Curve is used to
determine the unknown concentrations. See
Concentration Experiments for step-by-step instructions on
calculating concentrations and on using this dialog box.
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