Ocean Optics CHEM2000 - Manual (Page 16)

2: Using OOIChem Software
blank in the sampling region. Storing a reference spectrum is requisite before the software can calculate
absorbance, transmission, and relative irradiance spectra. This command merely stores a reference
spectrum. To permanently save the reference spectrum to disk, select File | Save Spectral Values from
the menu.
Dark Scan
Selecting the Dark button activates a prompt to make sure the light path is
blocked. You then must choose to either Store or Cancel your dark scan
. A dark
spectrum is taken with the light path to the spectrometer blocked. Storing a dark
spectrum is requisite before the software can calculate absorbance, transmission,
and relative irradiance spectra. This command merely stores a dark spectrum. To permanently save the
reference spectrum to disk, select File | Save Spectral Values from the menu.
Subtract Dark
Selecting this box subtracts the current dark spectrum from the spectra being displayed. This command is
useful if you are trying to look at a change in an emission spectrum or are trying to eliminate from the
spectra fixed pattern noise caused by a very long integration period. The subtract dark spectrum function
only acts on spectra displayed in Scope Mode.
Acquire Data Modes
Chart Active
When Spectrum & Kinetics is chosen as the Graph and chart display mode in
the Spectrometer Configuration dialog box, the Chart Active function becomes
visible in the display area above the Scan button. This function is responsible only
for the Kinetics chart. By deselecting this function, users can use the scan button
and collect data in just the spectrum section of the graph. If the function is
enabled, choosing the scan button results in the collection of data in both the
spectrum section of the graph and the kinetics section of the graph.
Scan/Stop Button
When in Single mode, the Scan button acts as a snapshot. After selecting the Single mode, click on the
Scan button to take a scan. The button depresses and Stop replaces Scan. The button will stay depressed
until the scan has been completed (the time set in the Integration Period box).
When in Continuous mode, the Scan button continuously takes scans. After each integration cycle,
another scan will immediately begin. The button depresses and Stop replaces Scan. Click on Stop to halt
the scanning process and discontinue acquiring data.
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