Ocean Optics CHEM2000 - Manual (Page 23)

Chapter 3
Experiment Tutorial
Overview
When you are ready to begin your experiment, you should have already installed the Maxwell and
OOIChem software. Now you are ready to take your measurements. Because of the components making
up your Maxwell system, it is ideal for absorbance and transmission. The Maxwell system can also make
relative irradiance measurements. If, however, you wish to use your system for other measuring functions,
additional products might be required. Contact an Ocean Optics Applications Scientist for options.
Absorbance Experiments
Absorbance spectra are a measure of how much light is absorbed by a sample. The software calculates
absorbance (
A λ ) using the following equation:
S
(
λ
-D
λ
A
λ
= - log
10
R λ
-D
λ
)
Where:
S = Sample intensity at wavelength λ
D = Dark intensity at wavelength λ
R = Reference intensity at wavelength λ
Common applications include the quantification of chemical concentrations in aqueous or gaseous
samples.
105-00000-CHM-02-0405 PRELIMINARY DRAFT 17