Olympus IX2-ARCEVA - Manual (Page 5)

IX2-ARCEVA
2
1 Getting Ready
1. This manual pertains only to the handling of the total reflection fluorescence illumination system. Please also read the
instruction manuals for the IX2 series (IX81/IX71/IX51) microscope and associated options to understand the comprehen-
sive operating instructions of the microscope system.
2. The total reflection fluorescence illumination system is composed of precision instruments. Handle it with care and avoid
subjecting it to sudden or severe impact.
3. Do not use the system where it is subjected to direct sunlight, high temperature and humidity, dust or vibrations.
4. Reserve spaces of 10 cm or more around the lamp housing and power supply to facilitate their ventilation.
5. The fluorescent light illuminator that can be used in combination with this system is only the IX2-RFAL L-shaped reflected
light illuminator.
6. The objectives that can be used with total reflection illumination are only the PlanApoN60XO
TIRFM
and PlanApo60XO
TIRFM
.
7. The illumination field of total reflection fluorescence observation is limited within the area of field number 11.
8. Since the total reflection light is not point-source light, the excitation light incidence angle on the specimen is not
constant and the effusion depth cannot be specified.
9. If an excitation filter is included in the mirror unit during total reflection fluorescence illumination, the repeated reflections
cause the leak of direct light to increase and degrade the contrast considerably. To prevent this, remove the excitation filter
from the mirror unit and mount in the excitation filter slider.
. The size of the excitation filter inserted in the filter slot of the excitation slider should be 25 mm in diameter and 6 mm or
less in thickness. Only one ND filter with a diameter of 32 mm and thickness of 1 mm or less can be inserted.
. When ordinary fluorescence observation is performed by inserting a 25 mm excitation filter in the excitation filter slider,
the illumination field is limited within the area of field number 20.
. To prevent light leak, be sure to attach the provided 32 mm light shields in the unused positions of the excitation filter
slider.
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