Olympus IX2-ARCEVA - Manual (Page 4)

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IMPORTANT
This system has been designed to enable total reflection fluorescence observation using an arc light source
(mercury burner or xenon burner).
Total reflection illumination is implemented by limiting the illumination projection area to the area corresponding
to the objective pupil NA of 1.39 (because the reflectance of cytoplasm is 1.38).
}This system offers the following advantages compared to the laser-based total reflection illumination system.
· Higher safety, because the laser light is not used.
· Easy handling without the care of interference stripe, because the light source is not a coherent source.
· Easy switching between multiple wavelengths.
Nevertheless, this system is not applicable to molecular fluorescence observation due to low intensity of the
illumination. Its applications are limited to high-contrast observations of tissues near cell membranes and
physiochemical phenomena.
SAFETY PRECAUTIONS
1. Do not ignite the mercury burner or xenon burner without mounting the lamp housing on the microscope.
The UV light emitted from the burner is hazardous for your eyes. Do not look directly at the light output from the lamp
housing.
For details precautions, also refer to the instruction manual for the light source.
2. When centering the burner or replacing the specimen, be sure to look them through the UV cut plate to protect your eyes.