Operation, 1 preparation – Olympus IX2-ARCEVA User Manual

Page 15

IX2-ARCEVA

1 2

OPERATION

4-1

Preparation

Centering the Burner

(Fig. 5)

}The following settings are the original settings required for this system.

The details may be variable according to the instruction manual for each
lamp housing.
@ Field iris diaphragm: Pulled-out position (Closed)
² Slit:

Pulled-out position (Slit OUT)

³ Wedge prism:

Nearest position (Vacant position)

| Excitation filter:

B or IB excitation filter engaged. Also the B or IB
mirror unit should be engaged in the light path.

Fig. 5

Adjusting the Slit

(Figs. 6 to 8)

}Open the field iris diaphragm @, set the slit selector lever ² to the OUT

position and set the wedge prism ³ to the vacant position before the
following adjustment. (Fig. 6)

1. Engage the PlanApoN60XO

TIRFM

or PlanApo60XO

TIRFM

objective (which re-

quires immersion oil) in the light path.

2. Place a fluorescent specimen on the stage and bring the specimen into

focus using general fluorescent light of B or IB.

3. Remove the specimen, rotate the revolving nosepiece and screw the

target into an objective position.

Fig. 6