Hudson Robotics SOLO - Manual (Page 43)

43
“ABC123”, then the file’s name would be “ABC123.csv”. The file contents are
simply the well name of each source well, followed by a comma then the amount
(uL) to be transferred into each target well. The format would looks as follows:
A01,50
C09,75 (each well,volume entry is on a separate line)
H11,55
The user can use a header line, if desired, of “Source,Well” for clarity. This line
is ignored by SOLOSoft if present. The HitPick Step allows the user to fill
multiple target wells from each source well, and the amount aspirated from the
source well will be that multiple of the amount in the source file. That is, if the
user elects to fill 3 target wells from each source well, the amount aspirated from
well “A01” above would be 150 uL, with SOLOSoft multiplying the amount in
the source file by 3. Each line in the output file has the format “Date,Time,Source
Plate Barcode,Source Well,Target Plate Barcode,Target Well,Amount (uL)”, with
no header line. A typical entry would appear as follows:
6/23/2008,2:40:24 PM,ABC123,A03,DEF456,A01,53
The picture below shows the HitPick Step’s graphic image:
The user can select a Maximum Volume per Aspiration to allow the use of pipette
tips that may be smaller than the amount needed to complete the transfer, and may
set the number of Target Wells per Source Well. The SOLO will only aspirate up
to the Max. Volume each time. If more than one Target Well is to be filled per
Source Well, the SOLO will divide that maximum volume by the number of