Hudson Robotics SOLO - Manual (Page 37)

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The tip box will be reset to “full” if the type of box at that location is changed, if
the identity of that tip box is changed (e.g., if operated using Hudson’s
SoftLinx scheduling software and a new tip box is moved into that position), or if
the tip box is reset in the “Change Plate Types” utility (see that subsection
below). In the step’s graphic plate image, available tips are shown in GREEN.
For a single-tip syringe type, each tip will be taken sequentially from the first
available position along rows starting with row H and progressing in direction
from column 12 to 1 and then rows H to A (for 384-well boxes, substitute “P” for
“H” and “24” for “12”).
For multi-tip syringe type ‘P8’ utilizing ‘single-tip’ option the single tip will be
taken sequentially from the first available position along columns starting with
column 12 and row H progressing in direction from row H to A and then columns
from 12 to 1. In case of using ‘multi-tip’ option, tips will be picked up from first
column with enough tips to meet the “Number of Tips to Use” entry, starting with
column 12.
For a multi-tip syringe type ‘P12’ utilizing ‘single-tip’ option the single tip will be
taken sequentially from the first available position along rows starting with row H
and progressing in direction from column 12 to 1 and then rows H to A. In case
of using ‘multi-tip’ option, tips will be picked up from the first row with enough
available tips, starting with row H.
For guidance on programming tip pickup positions and using different length
disposable tips in different methods, see the subsection below entitled
“Programming with Disposable Tips”.
3.5 – Shuck Tip Step
This step, whose GUI is shown in the next figure, allows the user to discard a
disposable tip at the position indicated in the “Select Disposal Point” box. This is
usually used at the very end of a method to strip off a remaining tip. Otherwise,
the “Get Tip” step is normally used to shuck a tip prior to getting another.