Bio-Rad Aurum™ Total RNA Fatty and Fibrous Tissue Kit - Manual (Page 7)

Section 3
Storage Conditions
Store components of the kit at the recommended temperatures (see Table 1
below).
Table 1. Recommended storage temperature for the Aurum™ total
RNA fatty and fibrous tissue kit components.
Kit Components Storage Temperature
PureZOL™ RNA isolation reagent Store at 4–25°C
Low stringency wash buffer Room temperature (before and after the
addition of ethanol)
High stringency wash buffer Room temperature
Elution solution Room temperature
DNase dilution solution 4°C; allow solution to equilibrate to
room temperature before use
DNase I (lyophilized) Room temperature in lyophilized form;
once reconstituted in 250 µl of 10 mM
Tris, pH 7.5, aliquot and store at –20°C
in a nonfrost-free freezer. Do not freeze-
thaw more than once after
reconstitution
Aurum RNA binding mini columns Room temperature
and microcentrifuge tubes
Section 4
Materials and Equipment Required
(Not Provided in the Kit)
• Microcentrifuge, capable of spinning at >12,000 x g at 4°C and room
temperature
• 95–100% ethanol, ACS grade or better
• Tris for DNase I reconstitution (catalog #161-0716)
• Chloroform (without additives such as isoamyl alcohol) for phase
separation; 0.2 ml of chloroform per 1 ml of PureZOL required
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