Bio-Rad Aurum™ Total RNA Fatty and Fibrous Tissue Kit - Manual (Page 15)

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Section 6
Vacuum Manifold Setup and Use With the
Column Adaptor Plate
Guidelines for Vacuum Format
• The recommended operating range is –17 to –20 inHg. Do not exceed
–25 inHg when performing this protocol. A vacuum regulator is strongly
recommended to establish the appropriate negative pressure.
Table 4. Pressure unit conversions.
To convert from inches of mercury (inHg) to: Multiply by:
millimeters of mercury or torr (mmHg, torr) 25.4
millibar (mbar) 33.85
atmospheres (atm) 0.03342
pounds per square inch (psi) 0.4912
kilopascals (kPa) 3.385
About the Column Adaptor Plate (CAP)
The Aurum™ CAP interfaces with the Aurum vacuum manifold to convert the
manifold from a plate-processing to a column-processing system. The CAP has
18 luer fittings in a 6 x 3 array and comes supplied with luer caps. Up to 18
Aurum RNA binding mini columns can be accommodated on the CAP without
the need for connectors or other manifold accessories. The CAP will also
accommodate other columns with luer ends.
When vacuum is applied to the manifold, the CAP should self-seat, forming
an airtight seal without the need to press it down. However, the application of
gentle downward force may occasionally be required to facilitate seating.
Preparing the Aurum™ Vacuum Manifold
Tubing provided in the Aurum vacuum manifold kit (catalog # 732-6470) is 4 ft
long and must be cut into appropriate pieces before proceeding.
Prior to setup, you may ensure that the gauge pointer is adjusted to zero by
removing the lens cover, followed by turning the adjustment pin located
beneath the dial face.
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