Bio-Rad Aurum™ Total RNA Fatty and Fibrous Tissue Kit - Manual (Page 3)

Table of Contents
Section 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . .1
Section 2 Kit Components . . . . . . . . . . . . . . . . . . . . . . . . .2
Section 3 Storage Conditions . . . . . . . . . . . . . . . . . . . . . . .3
Section 4 Materials and Equipment Required
(Not Provided in the Kit) . . . . . . . . . . . . . . . . . . .3
Supplies for Tissue Grinding, Disruption,
and Homogenization . . . . . . . . . . . . . . . . . . . . . . . . . . .4
Additional Equipment Required for Vacuum Format . . . .4
Section 5 Before Using the Aurum™
Total RNA Fatty and Fibrous Tissue Kit . . . . . . .5
Maximum Starting Material Amounts . . . . . . . . . . . . . . .5
Minimum Starting Material Amounts . . . . . . . . . . . . . . . .5
Reagents Used With the Aurum Total RNA
Fatty and Fibrous Tissue Kit . . . . . . . . . . . . . . . . . . . . . .7
Maintaining an RNase-Free Environment . . . . . . . . . . . .8
Sample Disruption and Homogenization . . . . . . . . . . . .9
Section 6 Vacuum Manifold Setup and Use With the
Column Adaptor Plate . . . . . . . . . . . . . . . . . . .11
Guidelines for Vacuum Format . . . . . . . . . . . . . . . . . . .11
About the Column Adapter Plate (CAP) . . . . . . . . . . . .11
Preparing the Aurum Vacuum Manifold . . . . . . . . . . . .11
Vacuum Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12
Manifold Wash Setup . . . . . . . . . . . . . . . . . . . . . . . . . .13
Section 7 Vacuum Protocol . . . . . . . . . . . . . . . . . . . . . . .14
Section 8 Spin Protocol . . . . . . . . . . . . . . . . . . . . . . . . . .20
Section 9 Troubleshooting Guide . . . . . . . . . . . . . . . . . . .25
Section 10 Ordering Information . . . . . . . . . . . . . . . . . . . .32
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