Bio-Rad Aurum™ Total RNA Fatty and Fibrous Tissue Kit - Manual (Page 35)

Problem Possible Cause Recommended Solution
Prepared total RNA RNA is degraded See troubleshooting
performs poorly section "RNA is
in downstream degraded"
applications
Ethanol contamination Make sure to perform the
of the eluate purge spin step (see
step 16) to remove
residual wash solution
prior to eluting the RNA
RNA is contaminated See troubleshooting
section "Low RNA
A
260
/
280
ratio"
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