Bio-Rad Aurum™ Total RNA Fatty and Fibrous Tissue Kit - Manual (Page 16)

12
Vacuum Setup (Figure 1)
1. Cut tubing into three pieces of appropriate length.
2. Use one piece of tubing to connect to the right side of the vacuum
regulator to the vacuum source.
3. Use another piece of tubing to connect the left side of the vacuum
regulator to the sidearm of the filter flask.
4. Place a rubber stopper with hole into the mouth of the filter flask. Insert
a serological pipette (or comparable) into the hole of the stopper.
5. Snap in the black sealed end of the quick connect fitting into the
manifold base.
6. Finally, use the last piece of tubing to connect the filter flask to the quick
connect fitting of the nozzle of the manifold.
Note: Use of the Aurum™ vacuum regulator is strongly recommended
to ensure full control of the negative pressure of the manifold.
Fig. 1. Vacuum setup conditions.
Vacuum manifold Filter flask Vacuum regulator Vacuum source
10001298B:4110133A.qxd 1/16/2009 2:40 PM Page 12