Bio-Rad Aurum™ Total RNA Fatty and Fibrous Tissue Kit User Manual
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Problem
Possible Cause
Recommended Solution
RNA binding mini
Starting material is
After the sample
column is clogging
high in fat, proteins,
disruption step,
(continued)
polysaccharides, or
centrifuge the lysate at
extracellular material,
12,000 x g for 10 min
causing RNA eluate
at 4°C to pellet any
impurities
debris present. Transfer
the supernatant into a
new 2.0 ml micro-
centrifuge tube, leaving
behind the pellet. Avoid
transferring the excess
fat that collects as a top
layer in lipid-rich
samples. Perform this
step before adding the
chloroform
Not enough vacuum
Make sure vacuum
pressure was applied
filtration steps are carried
for the filtrate to pass
out at –17 to –23 inHg
through the columns
for optimum performance.
Alternatively, transfer the
RNA binding columns to
a 2.0 ml capless tube
and centrifuge for 60 sec
at
>12,000 x g
Problems that may be encountered after RNA is eluted from the
column:
Low RNA yield
Excessive amount of
Do not exceed the
starting material
maximum starting
amount limit for the kit
(see Section 5). If
clogging occurs when
using the maximum
starting amount, reduce
the amount of material
used
Inefficient elution
Preheat the elution
solution to 70°C in water
bath prior to the elution
step
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2:40 PM
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