Bio-Rad Aurum™ Total RNA Fatty and Fibrous Tissue Kit - Manual (Page 31)

Problem Possible Cause Recommended Solution
Low RNA yield Low amount of Do not use less than the
(continued) starting material recommended minimum
starting amount (see
Section 5). When
processing small
amounts of starting
material (<500,000 cells
or <10 mg of tissue),
perform a single elution
with 30 µl of elution
solution
Incomplete disruption Increase the duration or
of starting material intensity of sample
that causes cells not disruption. Make sure
to be lysed, and thus that 1 ml of PureZOL is
fail to release RNA used per prep
into the lysate to be
recovered
Incorrect use of wash Add the required amount
solutions. (Incorrect of ethanol to the
ethanol concentration low stringency wash
in the low stringency buffer before use
wash can cause
accidental elution of
the RNA from the
membrane)
Incorrect DNase I Use only the DNase
dilution solution used, dilution solution included
causing accidental in the kit to dilute the
elution of the RNA DNase I
from the membrane
Elution solution was Avoid pipetting the
not pipetted directly elution solution onto the
onto the center of the side of the column or on
membrane top of the ring that holds
the membrane stack in
place
Elution contamination Prior to eluting the RNA,
of the eluate make sure to perform the
purge spin step (see step
16 in spin protocol) to
remove residual ethanol
in the wash solution
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