Bio-Rad Aurum™ Total RNA Fatty and Fibrous Tissue Kit - Manual (Page 10)

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yeast for processing. To calculate the volume of culture required, use the following
equation:
(OD
600
of undiluted culture) x (culture volume in ml) = # OD•ml
For example, 3 OD•ml of yeast would require 500 µl of an undiluted culture with an
OD
600
= 6.
Note: 1 OD
600
is equivalent to approximately 8 x 10 8 bacterial cells/ml, or
1 x 10 7 yeast cells/ml.
Table 2. Yield (per column) of total RNA from various samples using
the Aurum total RNA fatty and fibrous tissue kit.
Average Yield
Starting Material (Amount Used)* (µg)**
Cultured cells (1 x 10 7 )
293H 145
Bacteria (2.4 x 10 9 or 3 OD•ml)
E. coli 30
Yeast (3 x 10 7 or 3 OD•ml)
S. cerevisiae 64
Fatty animal tissue (100 mg)
Brain 96
Breast 58
Adipose 14
Fibrous animal tissue (100 mg)
Heart 85
Cartilage 54
Skin 63
Plant tissue (100 mg)
Potato 91
Arabidopsis 5
Filamentous fungi (50 mg)
Aspergillus niger 12
* Starting material amounts in parentheses are the maximum amounts recommended for this kit. Note: The
elution volume should be decreased to 30 µl if only a small amount of starting material (<500,000 cells or
<10 mg of animal or plant tissue) is used.
** Yield figures are representative of a minimum of 20 mini column preps performed in both vacuum and spin
formats.
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