Bio-Rad Aurum™ Total RNA Fatty and Fibrous Tissue Kit User Manual
Page 29

Section 9
Troubleshooting Guide
Problems that may be encountered during RNA purification:
Problem
Possible Cause
Recommended Solution
Incomplete
Lysate was not mixed
Once chloroform is
separation of
properly after adding
added, mix tubes
phases after
chloroform (see step 4
vigorously by shaking
centrifugation
in protocol)
for 15 sec. Do not vortex!
Let the lysate incubate for
5 min at room
temperature, mix again
before centrifuging
Lysate was not
Make sure that
centrifuged at the right
centrifugation step is
temperature
performed at 4°C
following the addition of
chloroform in order to
achieve complete
separation of the phases
Incorrect amount of
For every 1 ml of PureZOL™
chloroform was added
used, add 0.2 ml of
chloroform
RNA binding mini
Incomplete disruption of
Increase the duration or
column is clogging
starting material
intensity of sample
disruption. Make sure to
use the 1 ml of PureZOL
for each prep
Excessive amount of
Do not exceed the
starting material
maximum starting amount
limit for the kit (see
Section 5). If clogging
persists when using the
maximum starting
amount, reduce the
amount of material used
25
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Page 25
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