Bio-Rad Aurum™ Total RNA Fatty and Fibrous Tissue Kit - Manual (Page 14)

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Table 3. Disruption and homogenization methods.
Starting
Material
Disruption Method Homogenization Method
Animal tissue Grind tissue with a Pipetting up and down,
mortar and pestle rotor-stator homogenizer,
under liquid nitrogen, bead mill homogenizer, or
use a rotor-stator 18-gauge needle and syringe
homogenizer, bead
mill homogenizer, or
18-gauge needle
and syringe
Plant tissue Grind tissue with a Pipetting up and down,
mortar and pestle rotor-stator homogenizer,
under liquid nitrogen, bead mill homogenizer, or
use a rotor-stator 18-gauge needle and syringe
homogenizer, bead
mill homogenizer, or
18-gauge needle
and syringe
Cultured cells Pipet up and Pipetting up and down, or
down, or use 18-gauge 18-gauge needle and syringe
needle and syringe
Bacteria Rotor-stator Pipetting up and down,
homogenizer, bead rotor-stator homogenizer, or
mill homogenizer, bead mill homogenizer
pipet up and down,
or 18-gauge needle
and syringe
Yeast Rotor-stator Pipetting up and down,
homogenizer, bead rotor-stator homogenizer, or
mill homogenizer, or bead mill homogenizer
18-gauge needle
and syringe
• Mortar and pestle: freeze the tissue with liquid nitrogen, then grind it into a
fine powder under liquid nitrogen
• Pipet up and down: pass the lysate through a standard micropipet tip
several times
• 18-gauge needle and syringe: pass the lysate through the needle several
times
• Rotor-stator homogenizer: immerse the tip of the homogenizer into the
solution and homogenize for 30–60 sec
• For bead mill homogenizers, follow manufacturer's instructions
If column clogging occurs, switching to a more vigorous homogenization
method may lower the incidence of column clogging.
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