Bio-Rad Econo Gradient Pump Kits - Manual (Page 40)

Problem Possible Cause Solution
Flow rate not Pump tubing Replace tubing.
consistent damaged or
worn out
Platen adjustment Adjust the platen by turning
incorrect
it counter clockwise until it is
completely loosened, then
rotate three to five full turns
clockwise. See section 4.3,
Platen Adjustment.
Wrong tubing size Refer to Figure 4-1 for
selection
selected criteria.
Select proper ID tubing within
Setup.
Large change in Re-calibrate flow within Setup.
back pressure or
fluid viscosity
Restriction on inlet Detach the inlet tubing and
side
Gradient Mixer. Rinse with
deionized water.
Excessive Platen too tight Adjust the platen by turning
wear on tubing it counter clockwise until it is
completely loosened, then
rotate three to five full turns
clockwise. See section 4.3,
Platen Adjustment.
Troubleshooting
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