Bio-Rad Econo Gradient Pump Kits - Manual (Page 20)

System Operation
16
Screen 4. Pump Calibration by Measured Flow.
Pressing the left or right soft keys will start the pump for the selected
time. Collect the pump effluent in a graduated cylinder. During
calibration, the pump runs at half speed (12.5 RPM). Typical volumes
collected after a 2-minute run are 20 ml, 6.4 ml, 1.7 ml, and 700
µl for
tubing IDs of 3.2 mm, 1.6 mm, 0.8 mm, and 0.51 mm respectively.
The volume collected is entered in screen 5.
4 Start cal run
2m 20m
The time remaining in the calibration run will be displayed as shown
below. It is not necessary to run a calibration to completion.
Time = _min: _s
End ABORT
• End: Ends volume collection and continues to screen 5,
permitting flow rate calibration, before the timer reaches 0
minutes.
• Abort: Ends volume collection and returns to screen 1,
disallowing flow rate calibration.
Screen 5. Calibrated Volume.
When the calibration run ends, enter the volume of liquid that was
collected.
5 Volume ?
_ ml
Press Next to accept the entered value and advance to screen 6.
Press Cancel to return to screen 4 and repeat the calibration.
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