beautypg.com

Bio-Rad BioLogic QuadTec™ Detector and Components User Manual

Page 9

background image

1.4 PREPARING THE QUADTEC DETECTOR FOR OPERATION

1.4.1 Installation of the Flow Cell

The QuadTec detector is shipped with a “dummy” flow cell installed. Before operating the detector, you need
to install the biocompatible 3 mm PEEK flow cell. Refer to Figure 1-4 and the procedure below.

1.

Make sure the detector power is OFF.

2.

Loosen the two knurled screws on the front of the flow cell and gently pull them out. This allows the
flow cell housing to slide out.

3.

Remove the dummy cell by gently pulling it upward. Save the dummy cell in a secure place. It is
required for measuring signal and reference output values for the lamps.

4.

Insert the new flow cell and make sure that the engraved specifications point towards the user and
that the fixing hole on the back side of the cell meets the corresponding metal pin of the detector’s
housing.

5.

Slide the complete system towards the detector, insert the two knurled screws, and tighten them by
hand.

6.

Attach the 10-32 fingertight fittings and tubing to the inlet and outlet ports and use a syringe to rinse
the flow cell with 10–20 ml of 100% analytical grade methanol, followed by 5 ml DI water.

Figure 1-4. Installing the Flow Cell

INTRODUCTION

SYSTEM OVERVIEW

1-4

3

0

Link

Load

Prog

Hold 0

Run 1

Time

(min)

1

(nm)

2

(nm)

Edit Prog

Clear O

Events:

2

off 0 on /

Signal 1

[ au ]

Signal 2

[ au ]

Vie

w

Setup

GLP

AU

TO

ZER

O

SCAN

1

2

3

4

5

6

7

8

9

0

SLIDES

FLOW CELL

INLET

OUTLET

FLOW CELL HOUSING