Bio-Rad BioLogic QuadTec™ Detector and Components - Manual (Page 6)

1.0 GENERAL DESCRIPTION
The BioLogic QuadTec multi-wavelength UV/Vis detector adds versatility to your biologic chromatography
system. The QuadTec detector has a wavelength range of 190–370 nm using the standard deuterium lamp
and 370–740 nm with the optional halogen lamp. The standard deuterium lamp is required for the detection
of peptides, proteins, DNA and other nucleotides.
Wavelengths are selected through the use of a moveable grating monochromator with an accuracy of ±1 nm
in 1 nm steps. The bandwidth is 8 nm or better. When using the deuterium lamp for the monitoring of
wavelengths >380 nm, an automatic cutoff filter is activated. This filter suppresses secondary grating orders
at wavelengths > 380 nm and thus ensures the selectivity of the measurement. Because very little light of
wavelengths greater than 400 nm is emitted from a deuterium lamp, the halogen lamp is required for routine
detection above 380 nm.
Figure 1-1. BioLogic QuadTec UV/Vis Detector
When connected to the BioLogic DuoFlow system via an Instrument Control Module (ICM), four wavelengths
may be monitored simultaneously and displayed on the Biologic DuoFlow Controller. In this configuration, the
QuadTec is controlled from the DuoFlow Controller.
When connected to the Biologic HR system via two Signal Import Modules (SIM-HR), two wavelengths may
be monitored and displayed simultaneously. In this configuration, wavelength selection and zero baseline
functions must be performed from the QuadTec UV/Vis Detector faceplate.
In stand-alone mode, two simultaneous wavelengths may be output to a chart recorder. The SCAN function
provides you with spectral information of a sample over the entire wavelength range and data may be output
to a chart recorder. This feature is NOT supported by the BioLogic system software.
INTRODUCTION SYSTEM OVERVIEW
1-1
View
Link
GLP
Hold 0
Run 1 Time
(min) 1
(nm) 2
(nm)
Load
Prog Edit Prog
Clear 0 Events:
off 0 2
on/
Signal 1
[ au ]
Signal 2
[ au ]
Setup
AUTO
ZERO
SCAN
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